P-103: Enhancement of Colonization on Mouse Spermatogonial Stem Cell by Low Intensity Ultrasound Stimulation
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Abstract:
Background: Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis. New procedure such as sound wave especially low intensity ultrasound (LIUS) can be effective on increasing the number of cells. In this study we investigated the effect of LIUS stimulation on mouse SSCs. Materials and Methods: Isolated SSCs from neonate mice cultured in DMEM culture medium with 10% fetal bovine serum (FBS). In the first phase of study, temperature controlled by LIUS stimulation of plate containing culture medium and in the second phase, SSCs stimulated by LIUS with 2 different Intensity dose (100 and 200 mW/cm2) for one week and then SSCs colonization assessed at 7th day. Results: The LIUS treatment of mouse SSCs increased the number and diameter colonies of SSCs in experimental groups compared to the control group. Average number of colonies in 100, 200 mW/cm2 and control group were 20
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Journal title
volume 7 issue 3
pages 76- 76
publication date 2013-09-01
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